Incorporation of Tritiated Thymidine in the Cells of Caenorhabditis Briggsae (nematoda) Reared in Axenic Culture

[Methyl-14C]-choline incorporation into nude mice bearing tumor xenografts-correlation with [methyl-3H]-thymidine: A pilot study

Background: Many in vitro studies suggested that choline incorporation into many types of tumors is related to cell proliferation. Whether in vivo choline incorporation is related also to cell proliferation or not was my question. Therefore, the aim of this pilot study was to investigate the relationship of in vivo [methyl- 14C]-choline incorporation and in vivo [methyl-3H]- thymidine incorpora...

The Axenic Cultivation of Rhabditis briggsae Dougherty and Nigon, 1949 (Nematoda : Rhabditidae). I. Experiments with Chick Embryo Juice and Chemically Defined Media

Tritiated thymidine incorporation does not measure DNA synthesis in ribavirin-treated human cells.

When the incorporation of tritiated thymidine into acid insoluble material was measured, ribavirin appeared to be a potent inhibitor of DNA synthesis in KB cells and human lymphocytes. Inhibition was nearly 100-fold less, however, when DNA synthesis was measured by incorporation of phosphorus-32-labeled phosphate or by DNA fluorescence. The potent inhibition detected by incorporation of tritiat...

Studies on tritiated thymidine incorporation into DNA molecules by electron microscopic autoradiography.

The incorporation of tritiated thymidine into DNA molecules was studied by electr.:m microscopic autoradiography. To make autoradiogram in electron microscopic level, DNA was extracted from rat ascites hepatoma (AH 130) cells after in vitro incubation with tritiated thymidine. Extracted DNA samples were rotary shadowed with platinum palladium and covered with emulsion. Silver grains demonstrate...

Temperature-dependent behaviours are genetically variable in the nematode Caenorhabditis briggsae.

Temperature-dependent behaviours in Caenorhabditis elegans, such as thermotaxis and isothermal tracking, are complex behavioural responses that integrate sensation, foraging and learning, and have driven investigations to discover many essential genetic and neural pathways. The ease of manipulation of the Caenorhabditis model system also has encouraged its application to comparative analyses of...